Reactions of cycle:
| Step | Reactant | Product | Catalyzed by | |
| 1M | 2ATP + HCO3- + NH4+ | carbamoyl phosphate + 2ADP + Pi | CPS | |
| 2M | carbamoyl phosphate + ornithine | citrulline + Pi | OTC | |
| 3C | citrulline + aspartate + ATP | argininosuccinate + AMP + PPi | ASS | |
| 4C | argininosuccinate | Arg + fumarate | ASL | |
| 5C | Arg + H2O | ornithine + urea | ARG1 |
CPS1 stands for carbamoyl phosphate synthase 1 enzyme. OTC stands for ornithine transcarbamoylase. ASS stands for arginine succinate synthase. ASL stands for arginosuccinate lyase . ARG stands for arginase .the first two steps occur in mitochondria(M) and the next 3 steps occur in cytosol(C)
